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KMID : 0903619910320030394
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Journal of the Korean Society for Horticultural Science
1991 Volume.32 No. 3 p.394 ~ p.400
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Micropropagation of Gypsophila paniculata through Shoot Tip Culture in Vitro
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Abstract
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In order to establish mass propagation system of Gypsophila paniculata by in vitro shoot tip culture, effects of growth regulators and ratio of NO₃-N/NH₄-N on the multiplication and growth of shoot were evaluated. Among cytokinins, BA was the most effective in shoot multiplication at 0.5∼2.0㎎/ℓ concentration, however vitrified plantlets increased compared with kinetin or 2iP treatment. Combination of BA and IAA ranging of 0.1∼0.3㎎/ℓ was better in shoot growth than BA treatment alone, bnt addition of growth regulators to media increased the vitrification of plantlets than control. Increasing ratio of NO₃-N/NH₄ reduced number of vitrified plantlets and fresh/dry weight, while low NO₃-N/NH₄-N increased vitrified ones. Addition of 1.0㎎/ℓ IBA into the media was effective in root formation of regenerated shoot in vitro. Survival rate of more than 93% of regenerated plants transferred in vivo condition, were resulted in perlite or mixture of perlite and vermiculite(1 : 1).
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